移至主內容

莊秀美

莊秀美
教授
國立清華大學博士
生科大樓11樓1107室/蛋白質修飾與功能分析研究室
(04)22840896轉116
smchuang@dragon.nchu.edu.tw

研究領域

研究主題

  1. 癌細胞轉移是面對癌症一個重要課題,許多機制參與其中。本實驗室一個重要主題為GCIP抑癌蛋白如何在細胞中抑制癌化並抑制癌細胞轉移,本實驗室已透過cDNA microarray方式大量篩選與此抑癌基因蛋白有關聯的基因表現,在肺癌與胃癌的主題上做深入的分子層探討。
  2. 本實驗室與長期合作夥伴共同進行小分子抗癌藥物篩選,如利用生物資訊與電腦模擬方式,建立篩選平台,以多種人類不同癌細胞篩選有效抗癌藥物及多種有效組合,並進一步探討抗癌機制。
  3. 透過Ubiquitin-proteasome system (UPS)之蛋白降解機制是維持細胞恆定與正常生理功能之重要機制,許多癌症之發生是因為此系統發生變異,導致正常細胞走向癌化。蛋白降解途徑與細胞癌化之關聯為本實驗室重要研究主題

研究方向

  1. GCIP抑癌蛋白在癌細胞異常表現的分子機制
  2. GCIP抑癌蛋白負調控癌細胞基因轉錄分子機制
  3. 小分子藥物抗癌效果篩選與抗癌機制
  4. UPS 與致癌蛋白降解調控在癌細胞之探討
  5. TRIB3作為抗癌藥物治療標記之探討

教授科目

訊號傳遞與細胞週期調控

活體內蛋白質功能分析

蛋白質降解途徑特論

Ubiquitination與人類疾病特論

酵母菌模式之生物醫學應用

細胞週期分子調控特論

高等細胞生物學(合授課程)

分子細胞生物學(合授課程)

分子生物學概論 (合授課程)

生物醫學新知與應用(通識課程)

常見疾病與預防 (通識課程)
 

簡要經歷

國立中興大學生物醫學研究所教授 (2020.08~迄今)
國立中興大學生物科技發展中心研發推動組組長(2019.02~迄今)
國立中興大學生物醫學研究所副教授 (2012.2~2020.07)
國立中興大學生科院碩士在職專班導師(2010.9~2016.6)
國立中興大學生物醫學研究所助理教授 (2005.8~2012.1) 
美國University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School 生物化學研究所博士後研究員 (2002.9~2005.6)
國立清華大學生命科學系博士後研究員( 2000.5~2002.9 )
工業技術研究院化學工業研究所副研究員 (1991.8~1995.7) 

 

獲獎項目

台中榮民總醫院與八大院校96年度合作研究計畫聯合成果發表會口頭報告 第二名

學術著作

研究成果發表

(A) Published Papers in Refereed Journals

A. 代表著作

  1. Ruei-Yue Liang, Bang-Hung Liu, Chih-Jou Huang1, Kuan-Ting Lin, Chih-Chung Ko, Lin-Lun Huang, Bin Hsu, Chun-Ying Wu and Show-Mei Chuang* (2020) MEK2 is a critical modulating mechanism to down-regulate GCIP stability and function in can-cer cells. FASEB Journal 34(2), 1958-1969.
  2. Chung-Yun Yu, Bang-Hung Liu, Shao-Yi Tang, Ruei-Yue Liang, Keng-Hao Hsub, Show-Mei Chuang* (2019) HR23A-knockdown lung cancer cells exhibit epithelial-to-mesenchymal transition and gain stemness properties through increased Twist1 stability. BBA - Molecular Cell Research 1866, 1118537.
  3. Rong-Jaan Lin, I-Jung Wu, Jo-Yu Hong, Bang-Hung Liu, Ruei-Yue Liang, Tein-Ming Yuan and Show-Mei Chuang* (2018) Capsaicin-induced TRIB3 up-regulation promotes apoptosis in gastric cancer cells. Cancer Management and Research 10, 4237-4248.
  4. Chang, An-Chen Chang, Po-Chun Chen, Yu-Feng Lin, Chen-Ming Su, Ju-Fang Liu, Tien-Huang Lin, Show-Mei Chuang*, Chih-Hsin Tang* (2018) Osteoblast-secreted WISP-1 promotes adherence of prostate cancer cells to bone via the VCAM-1/integrin alpha4beta1 system. Cancer Letters 426, 47-56.
  5. I-Jung Wu, Rong-Jaan Lin, Hsin-Chiao Wang, Tein-Ming Yuan, Show-Mei Chuang* (2017) TRIB3 downregulation enhances doxorubicin-induced cytotoxicity in gastric cancer cells. Archives of Biochemistry and Biophysics 622, 26-35.
  6. Xiaotong Tan, Hsin-Chiao, Wang, Ruei-Yue Liang, Show-Mei Chuang* (2016) Human Rad23A plays a regulatory role in autophagy. Biochemical and Biophysical Research Communication 30;478(4):1772-1779.
  7. Tien-Sheng Tseng1, Show-Mei Chuang1, Nai-Wan Hsiao, Yi-Wen Chen, Yu-Ching Lee, Chi-Chen Lin, Cheng Huang, Keng-Chang Tsai (2016) Discovery of a potent cyclooxygenase-2 inhibitor, S4, through docking-based pharmacophore screening, in vivo and in vitro estimations. Molecular BioSystems 19;12(8):2541-2551.
  8. Yi-Hwa Lin1, Show-Mei Chuang1, Pei-Ching Wu, Chun-Liang Chen, Sivakamavalli Jeyachandran, Shou-Chen Lo, Hsu-Shan Huang, Ming-Hon Hou (2016) Selective recognition and stabilization of new ligands targeting the potassium form of the human telomeric G-quadruplex DNA. Scientific Reports 6, 31019.
  9. Xiaotong Tan, Ruei-Yue Liang, Show-Mei Chuang* (2015) hHR23A is required to control the basal turnover of Chk1. Cellular Signalling 27, 23042313.
  10. Ruei-Yue Liang, Hsin-Fang Tu, Xiaotong Tan,Yu-Shan Yeh, Pin Ju Chueh and Show-Mei Chuang* (2015) A gene signature for gold nanoparticle-exposed human cell lines. Toxicology Research 4, 365-375.
  11. Tein-Ming Yuan, Ruei-Yue Liang, Pin Ju Chueh and Show-Mei Chuang* (2015) Role of ribophorin II in the response to anticancer drugs in gastric cancer cell lines. Oncology Letters 9, 1861-1868.
  12. Ruei-Yue Liang, Li Chen, Bo-Ting Ko, Yu-Han Shen, Yen-Te Li, Bo-Rong Chen, Kuan-Ting Lin, Kiran Madura* and Show-Mei Chuang* (2014) Rad23 interaction with the proteasome is regulated by phosphorylation of its ubiquitin-like (UbL) domain. Journal of Molecular Biology 426, 4049-4060.
  13. Tein-Ming Yuan, Ruei-Yue Liang, Nai-Wan Hsiao and Show-Mei Chuang* (2014) The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells. Oncology Report 32, 2307-2318.
  14. Pin Ju Chueh, Ruei-Yue Liang, Yi-Hui Lee, Zih-Ming Zeng, Show-Mei Chuang* (2014) Differential cytotoxic effects of gold nanoparticles in different mammalian cell lines. Journal of Hazardous Materials 264, 303– 312.
  15. Show-Mei Chuang, Yi-Hui Lee, Ruei-Yue Liang, Gwo-Dong Roam, Zih-Ming Zeng, Hsin-Fang Tu, Shi-Kwun Wang, Pin Ju Chueh (2013) Extensive evaluations of the cytotoxic effects of gold nanoparticles. Biochimica et Biophysica Acta 1830, 4960–4973.
  16. Shan-Chi Liu, Chin-Jung Hsu, Yi-Chin Fong, Show-Mei Chuang*, Chih-Hsin Tang* (2013) CTGF induces monocyte chemoattractant protein-1 expression to enhance monocyte migration in human synovial fibroblasts. Biochimica et Biophysica Acta 1833, 11141124.
  17. Shan-Chi Liu, Chin-Jung Hsu, Hsien-Te Chen, Hsi-Kai Tsou, Show-Mei Chuang*, Chih-Hsin Tang* (2012) CTGF Increases IL-6 Expression in Human Synovial Fibroblasts through Integrin-Dependent Signaling Pathway. PLoS One 7(12):e51097.
  18. Show-Mei Chuang, Hsueh-Fang Wang, Ching-Chuan Hsiao, and Shur-Hueih Cherng (2012) Zinc Ion Enhances GABA Tea-Mediated Oxidative DNA Damage. Journal of Agricultural and Food Chemistry 60, 1586-1594.
  19. Yu-Han Shen, Bo-Rong Chen, Shur-Hueih Cherng, Pin Ju Chueh, Xiaoton Tan, Yun-Wei Lin, Jin-Ching Lin, and Show-Mei Chuang* (2011) Cisplatin transiently up-regulates hHR23 expression through enhanced translational efficiency in A549 adenocarcinoma cells. Toxicology Letters 205, 341-350.
  20. Tzou-Chi Huang, Yu-Ling Chun, Mei-Li Wu*, Show-Mei Chuang* (2011) Cinnamaldehyde Enhances Nrf2 Nuclear Translocation to Upregulate Phase II Detoxifying Enzyme Expression in HepG2 cells. Journal of Agricultural and Food Chemistry 59, 5164-5171.
  21. Shing Huang, Pin Ju Chueh, Yun-Wei Lin, Tung-Sheng Shih, Show-Mei Chuang* (2009) Disturbed Mitotic Progression and Genome Segregation Are Involved in Cell Transformation Mediated by Nano-TiO2 Long-Term Exposure. Toxicology and Applied Pharmacology 241, 182-194.
  22. Show-Mei Chuang, Lyu-Han Wang, Jhao-Hao Hon, Yun-Wei Lin (2008) Induction of Rad51 protein levels by p38 MAPK decreases cytotoxicity and mutagenicity in benzo[a]pyrene-exposed human lung cancer cells. Toxicology and Applied Pharmacology 230, 290-297.
  23. Show-Mei Chuang and Kiran Madura1 (2005) Saccharomyces cerevisiae Ub-conjugating enzyme Ubc4 binds the proteasome in the presence of translationally-damaged proteins. Genetics 171, 1477-1484.
  24. Show-Mei Chuang, Li Chen, David Lambertson, Monika Anand, Terri Goss Kinzy, and Kiran Madura (2005) Proteasome-mediated degradation of co-translationally damaged proteins involves the translation elongation factor eEF1A. Mol. Cell. Biol. 25, 403-413.
  25. Show-Mei Chuang, I-Ching Wang, Yi-Shi Hwua and Jia-Ling Yang (2003) Short-term depletion of catalase suppresses cadmium-elicited c-Jun N-terminal kinase activation and apoptosis: role of protein phosphatases. Carcinogenesis 24, 7-15.
  26. Show-Mei Chuang and Jia-Ling Yang (2001) Comparison of roles of three mitogen-activated protein kinases induced by chromium(VI) and cadmium in non-small-cell lung carcinoma cells. Molecular and Cellular Biochemistry 222, 85-95.
  27. Show-Mei Chuang, Geou-Yarh Liou and Jia-Ling Yang (2000) Activation of JNK, p38 and ERK mitogen-activated protein kinases by chromium(VI) is mediated through oxidative stress but does not affect cytotoxicity. Carcinogenesis 21, 1491-1500.  
  28. Show-Mei Chuang, I-Ching Wang and Jia-Ling Yang (2000) Roles of JNK, p38 and ERK mitogen-activated protein kinases in the growth inhibition and apoptosis induced by cadmium. Carcinogenesis 21, 1423-1432.
  29. Ming Chung Chang, Shwu Yuh Chang, Shing Li Cher and Show-Mei Chuang (1992) Cloning and expression in Escherichia coli of the gene encoding an extracellular deoxyribonuclease (DNase) from Aeromonas hydrophilia. Gene 122, 175-180.

 

B. 參考著作:

  1. Kai-Cheng Chuang, Chuang-Rung Chang, Shu-Hao Chang, Shi-Wei Huang, Show-Mei Chuang, Zheng-Yi Li, Sin-Ting Wang, Jun-Kai Kao, Yi-Ju Chen, Jeng-Jer Shieh (2020) Imiquimod-induced ROS production disrupts the balance of mitochondrial dynamics and increases mitophagy in skin cancer cells. Journal of Dermatological Science 98: 152-162.
  2. Yi-Hui Lee, Show-Mei Chuang, Sheng-Chi Huang, Xiaotong Tan, Ruei-Yue Liang, Gordon C C Yang, Chueh, Pin Ju (2017) Biocompatibility assessment of nanomaterials for environmental safety screening. Environmental Toxicology 32(4):1170-1182.
  3. S-C Liu, S-M Chuang, C-J Hsu, C-H Tsai, S-W Wang and C-H Tang* (2014) CTGF increases vascular endothelial growth factor-dependent angiogenesis in human synovial fibroblasts by increasing miR-210 expression. Cell Death and Disease 5, e1485.
  4. Shih-Ying YenJung-Kai TsengShow-Mei ChuangShuen-Ei ChenJyh-Cherng Ju (2014) Expression and Activation of Mitogen-activated Protein Kinases in Matured Porcine Oocytes under Thermal Stress. Journal of reproduction and development 60, 388-394.
  5. Chun-Wei Hsu, Chia-Feng Kuo, Show-Mei Chuang, Ming-Hon Hou (2013) Elucidation of the DNA-interacting properties and anticancer activity of a Ni(II)-coordinated mithramycin dimer complex. Biometals 26:1–12.
  6. Sheng-Yu Wang, Yueh-Luen Lee, Yi-Hua Lai, Jeremy J W Chen, Wen-Lin Wu, Jeu-Ming P Yuann, Wang-Lin Su, Show-Mei Chuang, Ming-Hon Hou (2012) Spermine attenuates the action of the DNA intercalator, actinomycin d, on DNA binding and the inhibition of transcription and DNA replication. PLoS One 7(11):e47101.
  7. Ching-Hsien Chen, Show-Mei Chuang, Meng-Fang Yang, Jiunn-Wang Liao, Sung-Liang Yu, and Jeremy J W Chen (2012) A Novel Function of YWHAZ/beta-Catenin Axis in Promoting Epithelial-Mesenchymal Transition and Lung Cancer Metastasis. Molecular Cancer Research 10, 1319-1221.
  8. Chun-Wei Hsu, Show-Mei Chuang, Wen-Ling Wu, and Ming-Hon Hou (2012) The Crucial Role of Divalent Metal Ions in the DNA-Acting Efficacy and Inhibition of the Transcription of Dimeric Chromomycin A3. PLoS One 7(9): e43792.
  9. Zih-Ming Zeng, Show-Mei Chuang, Ting-Chia Chang, Chen-Wei Hong, Jou-Chun Chou, Jaw-Ji Yang, Pin Ju Chueh (2012) Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells. Experimental Cell Research 318, 1759-1766.
  10. Shan-Chi Liu, Show-Mei Chuang, Chih-Hsin Tang (2012) d-pinitol inhibits RANKL-induced osteoclastogenesis. International Immunopharmacology 12, 494-500.
  11. His-Ming Wang, Show-Mei Chuang, Yu-Ching Su,Yi-Hui Li, and Pin Ju Chueh (2011) Down-Regulation of Tumor-Associated NADH Oxidase, tNOX (ENOX2), Enhances Capsaicin-Induced Inhibition of Gastric Cancer Cell Growth. Cell Biochemistry and Biophysics 61, 355-366.
  12. Hsueh-Fang Wang, Show-Mei Chuang, Ching-Chuan Hsiao, and Shur-Hueih Cherng (2011) A Synergistic Effect of GABA Tea and Copper(ii) on DNA Breakage in Human Peripheral Lymphocytes. Food and Chemical Toxicology 49, 955-962.
  13. Li Chen, Lizbeth Romero, Show-Mei Chuang, Vincent Tournier, Kishore Kumar Joshi, Jung Ah Lee, Gopala Kovvali and Kiran Madura (2011) STS1 Plays A Key Role in Targeting Proteasomes to The Nucleus. Journal of Biological Chemistry 286, 3104-3118.
  14. Ching-Hsien Chen, Ho Lin, Show-Mei Chuang, Sheng-Yi Lin and Jeremy J.W. Chen. (2010) Acidic stress facilitates tyrosine phosphorylation of HLJ1 to associate with actin cytoskeleton in lung cancer cells.  Experimental Cell Research 316, 2910-2921.
  15. Jen-Chung Ko, Shih-Ci Ciou, Jhih-Yuan Jhan, Chao-Min Cheng, Ying-Jhen Su, Show-Mei Chuang, Szu-Ting Lin, Chia-Che Chang, and Yun-Wei Lin. (2009) Roles of MKK1/2-ERK1/2 and Phosphoinositide 3-Kinase–AKT Signaling Pathways in Erlotinib-Induced Rad51 Suppression and Cytotoxicity in Human Non–Small Cell Lung Cancer Cells. Mol Cancer Res 7, 1378-1389.
  16. Ruey-Shyang Chen, Jhih-Yuan Jhan, Ying-Jhen Su, Wei-Ting Lee, Chao-Min Cheng, Shih-Ci Ciou, Szu-Ting Lin, Show-Mei Chuang, Jen-Chung Ko, Yun-Wei Lin (2009) Emodin enhances gefitinib-induced cytotoxicity via Rad51 downregulation and ERK1/2 inactivation. Experimental Cell Research 315, 26582672.
  17. Tsing-Fen Ho, Yu-Ta Peng, Show-Mei Chuang, Shin-Chang Lin, Bo-Lin Feng, Chien-Hsing Lu, Wan-Ju Yu, Jo-Shu Chang, Chia-Che Chang (2009) Prodigiosin down-regulates survivin to facilitate paclitaxel sensitization in human breast carcinoma cell lines. Toxicology and Applied Pharmacology 235, 253260.
  18. Yun-Wei Lin, Show-Mei Chuang, and Jia-Ling Yang (2003) ERK1/2 Achieves Sustained Activation by Stimulating MAPK Phosphatase-1 Degradation via the Ubiquitin-Proteasome Pathway. Journal of Biological Chemistry 278, 21534-21541.
  19. Yun-Wei Lin, Show-Mei Chuang and Jia-Ling Yang (2003) Persistent activation of ERK1/2 by lead acetate increases nucleotide excision repair synthesis and confers anti-cytotoxicity and anti-mutagenicity. Carcinogenesis 24, 53-61. (NSC90-2311-B-007-007)

 (B) Published abstracts in international meetings

  1. Mei-Li Wu, Show-Mei Chuang, Ru-Ling Jiang and Hso-Chi Chaung2011Application of a Surface Displayed Receptor Platform for Screening Biological Ligands of Cannabinoid Receptor 2  International Conference on Bioscience, Biochemistry and Bioinformatics - ICBBB 2011, Singapore, Feb 26-28.
  2. Kuan-Ting Lin, Ming-Chung Chang, Show-Mei Chuang* (2010). MEK/ERK signaling regulates the GCIP expression to control metastasis in human non-small cell lung carcinoma cells. 8th AACR-JCA Joint conference, Waikoloa, Hawaii, USA, February 5-9, 2010.
  3. Shing Huang, Pin Ju Chueh, Yun-Wei Lin, Tung-Sheng Shih, Show-Mei Chuang* (2009) Long-term Exposure to nano-TiO2 Promotes Genomic Instability and Cell Transformation in Mammalian Cells. 2009 AACR annual meeting, Denver, NC, USA, April 17-22, 2009.
  4. Show-Mei Chuang, Lyu-Han Wang, Jhao-Hao Hon, Yun-Wei Lin (2008) B[a]p increases Rad51 level and prevents cytotoxicity and mutagenicity in human lung cancer cells. 2008 AACR annual meeting, San Diego, CA, USA, April 12-16, 2008.
  5. Yun-Wei Lin, Chao-Min Cheng, Jen-Chung Ko, Shih-Ci Ciou, Jhih-Yuan Jhan, Ying-Jhen Su, Szu-Ting Lin, and Show-Mei Chuang* (2008) Role of MKK1/2-ERK1/2 and PI3K-AKT signaling in erlotinib decrease Rad51 expression and cell survival in human non-small cell lung cancer cells. 2008 AACR annual meeting, San Diego, CA, USA, April 12-16, 2008.
  6. Huang, S., Chuang, S.-M., Huang, Y-S., and Cheuh, P-J. (2007) Investigation of biological effects of TiO2 nanparticles on mammalian NIH3T3 cells. 3rd International Symposium on Nantechnology, Occupational and Environmental Health. Taipei, Taiwan, Aug. 29 - Sep 1, 2007.
  7. Liu, S., Chuang, S. M., and Chueh, P. J. (2006) The Biological Function of tNOX in Cell Migration by RNAi-mediated Gene Silencing. The American Society for Cell Biology, USA, San Diego, December 8-13.
  8. Kiran Madura, David Lambertson, Show-Mei Chuang, Tatiana G. Ortolan, Li Chen, and Lizbeth Romero-Perez (2004) Genetic studies reveal insight into Rad23 function. FASEB journal 18(8): C309-C309 Suppl. S, May 14, 2004.
  9. Chuang, S.-M., and Yang, J.-L. (2001) Involvement of catalase in regulation of c-JUN N-terminal kinase activation. Mutation Research 483 (Suppl. 1) S139. (8th International Conference on Environmental Mutagens. Shizuoka, Japan, October 21-26, 2001.
  10. Yang, J.-L., Liou, G.-Y., Chuang, S.-M., and Lin, Y.-W. (2001) Roles of three mitogen-activated protein kinases in UV-induced mutagenesis. Mutation Research 483 (Suppl. 1) S89. (8th International Conference on Environmental Mutagens. Shizuoka, Japan, October 21-26, 2001)
  11. Chuang, S.-M., Yang J.-L. (1998) Roles of reactive oxygen species in the activation of JNK, p38, and ERK by chromium(VI) in non-small-cell lung carcinoma cells. Cancer Detection and Prevention 22 (Suppl. 1), Page S-60 (4th International Symposium on Predictive Oncology and Therapy. Nice, France, October 24-27, 1998)
  12. Chuang, S.-M., Wang, I.-C, Hwua, Y.-S., Yang, J.-L. (1997) Role of JNK activation in genotoxicity induced by cadmium chloride in mammalian cells. Page 34. (American Cancer Society 8th Annual Research Conference. East Lansing, Michigan, September 12-13, 1997)
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