Show-Mei Chuang

Show-Mei Chuang
Professor
PhD,National Tsing Hua University
生科大樓11樓1107室/蛋白質修飾與功能分析研究室
(04)22840896轉116,117
smchuang@dragon.nchu.edu.tw

Research areas

研究主題

  1. 癌細胞轉移是面對癌症一個重要課題，許多機制參與其中。本實驗室一個重要主題為GCIP抑癌蛋白如何在細胞中抑制癌化並抑制癌細胞轉移，本實驗室已透過cDNA microarray方式大量篩選與此抑癌基因蛋白有關聯的基因表現，在肺癌與胃癌的主題上做深入的分子層探討。
  2. 本實驗室與長期合作夥伴共同進行小分子抗癌藥物篩選，如利用生物資訊與電腦模擬方式，建立篩選平台，以多種人類不同癌細胞篩選有效抗癌藥物及多種有效組合，並進一步探討抗癌機制。
  3. 透過Ubiquitin-proteasome system (UPS)之蛋白降解機制是維持細胞恆定與正常生理功能之重要機制，許多癌症之發生是因為此系統發生變異，導致正常細胞走向癌化。蛋白降解途徑與細胞癌化之關聯為本實驗室重要研究主題

研究方向

  1. GCIP抑癌蛋白在癌細胞異常表現的分子機制
  2. GCIP抑癌蛋白負調控癌細胞基因轉錄分子機制
  3. 小分子藥物抗癌效果篩選與抗癌機制
  4. UPS 與致癌蛋白降解調控在癌細胞之探討
  5. TRIB3作為抗癌藥物治療標記之探討

Courses offering

訊號傳遞與細胞週期調控

活體內蛋白質功能分析

蛋白質降解途徑特論
Ubiquitination與人類疾病特論

酵母菌模式之生物醫學應用

細胞週期分子調控特論

高等細胞生物學(合授課程)

分子細胞生物學(合授課程)

分子生物學概論 (合授課程)

生物醫學新知與應用(通識課程)

常見疾病與預防 (通識課程)
 

Work Experience

國立中興大學生物醫學研究所教授 (2020.08~迄今)
國立中興大學生物科技發展中心研發推動組組長(2019.02~迄今) 
國立中興大學生物醫學研究所副教授 (2012.2~2020.07)
國立中興大學生科院碩士在職專班導師(2010.9~2016.6)
國立中興大學生物醫學研究所助理教授 (2005.8~2012.1) 
美國University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School 生物化學研究所博士後研究員 (2002.9~2005.6)
國立清華大學生命科學系博士後研究員( 2000.5~2002.9 )
工業技術研究院化學工業研究所副研究員 (1991.8~1995.7) 

Award

台中榮民總醫院與八大院校96年度合作研究計畫聯合成果發表會口頭報告 第二名

Academic Works

研究成果發表

(A) Published Papers in Refereed Journals

A. 代表著作

  1. Ruei-Yue Liang, Bang-Hung Liu, Chih-Jou Huang1, Kuan-Ting Lin, Chih-Chung Ko, Lin-Lun Huang, Bin Hsu, Chun-Ying Wu and Show-Mei Chuang* (2020) MEK2 is a critical modulating mechanism to down-regulate GCIP stability and function in can-cer cells. FASEB Journal 34(2), 1958-1969.
  2. Chung-Yun Yu, Bang-Hung Liu, Shao-Yi Tang, Ruei-Yue Liang, Keng-Hao Hsub, Show-Mei Chuang* (2019) HR23A-knockdown lung cancer cells exhibit epithelial-to-mesenchymal transition and gain stemness properties through increased Twist1 stability. BBA - Molecular Cell Research 1866, 1118537.
  3. Rong-Jaan Lin, I-Jung Wu, Jo-Yu Hong, Bang-Hung Liu, Ruei-Yue Liang, Tein-Ming Yuan and Show-Mei Chuang* (2018) Capsaicin-induced TRIB3 up-regulation promotes apoptosis in gastric cancer cells. Cancer Management and Research 10, 4237-4248.
  4. Chang, An-Chen Chang, Po-Chun Chen, Yu-Feng Lin, Chen-Ming Su, Ju-Fang Liu, Tien-Huang Lin, Show-Mei Chuang*, Chih-Hsin Tang* (2018) Osteoblast-secreted WISP-1 promotes adherence of prostate cancer cells to bone via the VCAM-1/integrin alpha4beta1 system. Cancer Letters 426, 47-56.
  5. I-Jung Wu, Rong-Jaan Lin, Hsin-Chiao Wang, Tein-Ming Yuan, Show-Mei Chuang* (2017) TRIB3 downregulation enhances doxorubicin-induced cytotoxicity in gastric cancer cells. Archives of Biochemistry and Biophysics 622, 26-35.
  6. Xiaotong Tan, Hsin-Chiao, Wang, Ruei-Yue Liang, Show-Mei Chuang* (2016) Human Rad23A plays a regulatory role in autophagy. Biochemical and Biophysical Research Communication 30;478(4):1772-1779.
  7. Tien-Sheng Tseng1, Show-Mei Chuang1, Nai-Wan Hsiao, Yi-Wen Chen, Yu-Ching Lee, Chi-Chen Lin, Cheng Huang, Keng-Chang Tsai (2016) Discovery of a potent cyclooxygenase-2 inhibitor, S4, through docking-based pharmacophore screening, in vivo and in vitro estimations. Molecular BioSystems 19;12(8):2541-2551.
  8. Yi-Hwa Lin1, Show-Mei Chuang1, Pei-Ching Wu, Chun-Liang Chen, Sivakamavalli Jeyachandran, Shou-Chen Lo, Hsu-Shan Huang, Ming-Hon Hou (2016) Selective recognition and stabilization of new ligands targeting the potassium form of the human telomeric G-quadruplex DNA. Scientific Reports 6, 31019.
  9. Xiaotong Tan, Ruei-Yue Liang, Show-Mei Chuang* (2015) hHR23A is required to control the basal turnover of Chk1. Cellular Signalling 27, 2304–2313.
  10. Ruei-Yue Liang, Hsin-Fang Tu, Xiaotong Tan,Yu-Shan Yeh, Pin Ju Chueh and Show-Mei Chuang* (2015) A gene signature for gold nanoparticle-exposed human cell lines. Toxicology Research 4, 365-375.
  11. Tein-Ming Yuan, Ruei-Yue Liang, Pin Ju Chueh and Show-Mei Chuang* (2015) Role of ribophorin II in the response to anticancer drugs in gastric cancer cell lines. Oncology Letters 9, 1861-1868.
  12. Ruei-Yue Liang, Li Chen, Bo-Ting Ko, Yu-Han Shen, Yen-Te Li, Bo-Rong Chen, Kuan-Ting Lin, Kiran Madura* and Show-Mei Chuang* (2014) Rad23 interaction with the proteasome is regulated by phosphorylation of its ubiquitin-like (UbL) domain. Journal of Molecular Biology 426, 4049-4060.
  13. Tein-Ming Yuan, Ruei-Yue Liang, Nai-Wan Hsiao and Show-Mei Chuang* (2014) The S100A4 D10V polymorphism is related to cell migration ability but not drug resistance in gastric cancer cells. Oncology Report 32, 2307-2318.
  14. Pin Ju Chueh, Ruei-Yue Liang, Yi-Hui Lee, Zih-Ming Zeng, Show-Mei Chuang* (2014) Differential cytotoxic effects of gold nanoparticles in different mammalian cell lines. Journal of Hazardous Materials 264, 303– 312.
  15. Show-Mei Chuang, Yi-Hui Lee, Ruei-Yue Liang, Gwo-Dong Roam, Zih-Ming Zeng, Hsin-Fang Tu, Shi-Kwun Wang, Pin Ju Chueh (2013) Extensive evaluations of the cytotoxic effects of gold nanoparticles. Biochimica et Biophysica Acta 1830, 4960–4973.
  16. Shan-Chi Liu, Chin-Jung Hsu, Yi-Chin Fong, Show-Mei Chuang*, Chih-Hsin Tang* (2013) CTGF induces monocyte chemoattractant protein-1 expression to enhance monocyte migration in human synovial fibroblasts. Biochimica et Biophysica Acta 1833, 1114–1124.
  17. Shan-Chi Liu, Chin-Jung Hsu, Hsien-Te Chen, Hsi-Kai Tsou, Show-Mei Chuang*, Chih-Hsin Tang* (2012) CTGF Increases IL-6 Expression in Human Synovial Fibroblasts through Integrin-Dependent Signaling Pathway. PLoS One 7(12):e51097.
  18. Show-Mei Chuang, Hsueh-Fang Wang, Ching-Chuan Hsiao, and Shur-Hueih Cherng (2012) Zinc Ion Enhances GABA Tea-Mediated Oxidative DNA Damage. Journal of Agricultural and Food Chemistry 60, 1586-1594.
  19. Yu-Han Shen, Bo-Rong Chen, Shur-Hueih Cherng, Pin Ju Chueh, Xiaoton Tan, Yun-Wei Lin, Jin-Ching Lin, and Show-Mei Chuang* (2011) Cisplatin transiently up-regulates hHR23 expression through enhanced translational efficiency in A549 adenocarcinoma cells. Toxicology Letters 205, 341-350.
  20. Tzou-Chi Huang, Yu-Ling Chun, Mei-Li Wu*, Show-Mei Chuang* (2011) Cinnamaldehyde Enhances Nrf2 Nuclear Translocation to Upregulate Phase II Detoxifying Enzyme Expression in HepG2 cells. Journal of Agricultural and Food Chemistry 59, 5164-5171.
  21. Shing Huang, Pin Ju Chueh, Yun-Wei Lin, Tung-Sheng Shih, Show-Mei Chuang* (2009) Disturbed Mitotic Progression and Genome Segregation Are Involved in Cell Transformation Mediated by Nano-TiO2 Long-Term Exposure. Toxicology and Applied Pharmacology 241, 182-194.
  22. Show-Mei Chuang, Lyu-Han Wang, Jhao-Hao Hon, Yun-Wei Lin (2008) Induction of Rad51 protein levels by p38 MAPK decreases cytotoxicity and mutagenicity in benzo[a]pyrene-exposed human lung cancer cells. Toxicology and Applied Pharmacology 230, 290-297.
  23. Show-Mei Chuang and Kiran Madura1 (2005) Saccharomyces cerevisiae Ub-conjugating enzyme Ubc4 binds the proteasome in the presence of translationally-damaged proteins. Genetics 171, 1477-1484.
  24. Show-Mei Chuang, Li Chen, David Lambertson, Monika Anand, Terri Goss Kinzy, and Kiran Madura (2005) Proteasome-mediated degradation of co-translationally damaged proteins involves the translation elongation factor eEF1A. Mol. Cell. Biol. 25, 403-413.
  25. Show-Mei Chuang, I-Ching Wang, Yi-Shi Hwua and Jia-Ling Yang (2003) Short-term depletion of catalase suppresses cadmium-elicited c-Jun N-terminal kinase activation and apoptosis: role of protein phosphatases. Carcinogenesis 24, 7-15.
  26. Show-Mei Chuang and Jia-Ling Yang (2001) Comparison of roles of three mitogen-activated protein kinases induced by chromium(VI) and cadmium in non-small-cell lung carcinoma cells. Molecular and Cellular Biochemistry 222, 85-95.
  27. Show-Mei Chuang, Geou-Yarh Liou and Jia-Ling Yang (2000) Activation of JNK, p38 and ERK mitogen-activated protein kinases by chromium(VI) is mediated through oxidative stress but does not affect cytotoxicity. Carcinogenesis 21, 1491-1500.  
  28. Show-Mei Chuang, I-Ching Wang and Jia-Ling Yang (2000) Roles of JNK, p38 and ERK mitogen-activated protein kinases in the growth inhibition and apoptosis induced by cadmium. Carcinogenesis 21, 1423-1432.
  29. Ming Chung Chang, Shwu Yuh Chang, Shing Li Cher and Show-Mei Chuang (1992) Cloning and expression in Escherichia coli of the gene encoding an extracellular deoxyribonuclease (DNase) from Aeromonas hydrophilia. Gene 122, 175-180.

 

B. 參考著作：

  1. Kai-Cheng Chuang, Chuang-Rung Chang, Shu-Hao Chang, Shi-Wei Huang, Show-Mei Chuang, Zheng-Yi Li, Sin-Ting Wang, Jun-Kai Kao, Yi-Ju Chen, Jeng-Jer Shieh (2020) Imiquimod-induced ROS production disrupts the balance of mitochondrial dynamics and increases mitophagy in skin cancer cells. Journal of Dermatological Science 98: 152-162.
  2. Yi-Hui Lee, Show-Mei Chuang, Sheng-Chi Huang, Xiaotong Tan, Ruei-Yue Liang, Gordon C C Yang, Chueh, Pin Ju (2017) Biocompatibility assessment of nanomaterials for environmental safety screening. Environmental Toxicology 32(4):1170-1182.
  3. S-C Liu, S-M Chuang, C-J Hsu, C-H Tsai, S-W Wang and C-H Tang* (2014) CTGF increases vascular endothelial growth factor-dependent angiogenesis in human synovial fibroblasts by increasing miR-210 expression. Cell Death and Disease 5, e1485.
  4. Shih-Ying Yen, Jung-Kai Tseng, Show-Mei Chuang, Shuen-Ei Chen, Jyh-Cherng Ju (2014) Expression and Activation of Mitogen-activated Protein Kinases in Matured Porcine Oocytes under Thermal Stress. Journal of reproduction and development 60, 388-394.
  5. Chun-Wei Hsu, Chia-Feng Kuo, Show-Mei Chuang, Ming-Hon Hou (2013) Elucidation of the DNA-interacting properties and anticancer activity of a Ni(II)-coordinated mithramycin dimer complex. Biometals 26:1–12.
  6. Sheng-Yu Wang, Yueh-Luen Lee, Yi-Hua Lai, Jeremy J W Chen, Wen-Lin Wu, Jeu-Ming P Yuann, Wang-Lin Su, Show-Mei Chuang, Ming-Hon Hou (2012) Spermine attenuates the action of the DNA intercalator, actinomycin d, on DNA binding and the inhibition of transcription and DNA replication. PLoS One 7(11):e47101.
  7. Ching-Hsien Chen, Show-Mei Chuang, Meng-Fang Yang, Jiunn-Wang Liao, Sung-Liang Yu, and Jeremy J W Chen (2012) A Novel Function of YWHAZ/beta-Catenin Axis in Promoting Epithelial-Mesenchymal Transition and Lung Cancer Metastasis. Molecular Cancer Research 10, 1319-1221.
  8. Chun-Wei Hsu, Show-Mei Chuang, Wen-Ling Wu, and Ming-Hon Hou (2012) The Crucial Role of Divalent Metal Ions in the DNA-Acting Efficacy and Inhibition of the Transcription of Dimeric Chromomycin A3. PLoS One 7(9): e43792.
  9. Zih-Ming Zeng, Show-Mei Chuang, Ting-Chia Chang, Chen-Wei Hong, Jou-Chun Chou, Jaw-Ji Yang, Pin Ju Chueh (2012) Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells. Experimental Cell Research 318, 1759-1766.
  10. Shan-Chi Liu, Show-Mei Chuang, Chih-Hsin Tang (2012) d-pinitol inhibits RANKL-induced osteoclastogenesis. International Immunopharmacology 12, 494-500.
  11. His-Ming Wang, Show-Mei Chuang, Yu-Ching Su,Yi-Hui Li, and Pin Ju Chueh (2011) Down-Regulation of Tumor-Associated NADH Oxidase, tNOX (ENOX2), Enhances Capsaicin-Induced Inhibition of Gastric Cancer Cell Growth. Cell Biochemistry and Biophysics 61, 355-366.
  12. Hsueh-Fang Wang, Show-Mei Chuang, Ching-Chuan Hsiao, and Shur-Hueih Cherng (2011) A Synergistic Effect of GABA Tea and Copper(ii) on DNA Breakage in Human Peripheral Lymphocytes. Food and Chemical Toxicology 49, 955-962.
  13. Li Chen, Lizbeth Romero, Show-Mei Chuang, Vincent Tournier, Kishore Kumar Joshi, Jung Ah Lee, Gopala Kovvali and Kiran Madura (2011) STS1 Plays A Key Role in Targeting Proteasomes to The Nucleus. Journal of Biological Chemistry 286, 3104-3118.
  14. Ching-Hsien Chen, Ho Lin, Show-Mei Chuang, Sheng-Yi Lin and Jeremy J.W. Chen. (2010) Acidic stress facilitates tyrosine phosphorylation of HLJ1 to associate with actin cytoskeleton in lung cancer cells.  Experimental Cell Research 316, 2910-2921.
  15. Jen-Chung Ko, Shih-Ci Ciou, Jhih-Yuan Jhan, Chao-Min Cheng, Ying-Jhen Su, Show-Mei Chuang, Szu-Ting Lin, Chia-Che Chang, and Yun-Wei Lin. (2009) Roles of MKK1/2-ERK1/2 and Phosphoinositide 3-Kinase–AKT Signaling Pathways in Erlotinib-Induced Rad51 Suppression and Cytotoxicity in Human Non–Small Cell Lung Cancer Cells. Mol Cancer Res 7, 1378-1389.
  16. Ruey-Shyang Chen, Jhih-Yuan Jhan, Ying-Jhen Su, Wei-Ting Lee, Chao-Min Cheng, Shih-Ci Ciou, Szu-Ting Lin, Show-Mei Chuang, Jen-Chung Ko, Yun-Wei Lin (2009) Emodin enhances gefitinib-induced cytotoxicity via Rad51 downregulation and ERK1/2 inactivation. Experimental Cell Research 315, 2658 – 2672.
  17. Tsing-Fen Ho, Yu-Ta Peng, Show-Mei Chuang, Shin-Chang Lin, Bo-Lin Feng, Chien-Hsing Lu, Wan-Ju Yu, Jo-Shu Chang, Chia-Che Chang (2009) Prodigiosin down-regulates survivin to facilitate paclitaxel sensitization in human breast carcinoma cell lines. Toxicology and Applied Pharmacology 235, 253–260.
  18. Yun-Wei Lin, Show-Mei Chuang, and Jia-Ling Yang (2003) ERK1/2 Achieves Sustained Activation by Stimulating MAPK Phosphatase-1 Degradation via the Ubiquitin-Proteasome Pathway. Journal of Biological Chemistry 278, 21534-21541.
  19. Yun-Wei Lin, Show-Mei Chuang and Jia-Ling Yang (2003) Persistent activation of ERK1/2 by lead acetate increases nucleotide excision repair synthesis and confers anti-cytotoxicity and anti-mutagenicity. Carcinogenesis 24, 53-61. (NSC90-2311-B-007-007)

 (B) Published abstracts in international meetings

  1. Mei-Li Wu, Show-Mei Chuang, Ru-Ling Jiang and Hso-Chi Chaung（2011）Application of a Surface Displayed Receptor Platform for Screening Biological Ligands of Cannabinoid Receptor 2  International Conference on Bioscience, Biochemistry and Bioinformatics - ICBBB 2011, Singapore, Feb 26-28.
  2. Kuan-Ting Lin, Ming-Chung Chang, Show-Mei Chuang* (2010). MEK/ERK signaling regulates the GCIP expression to control metastasis in human non-small cell lung carcinoma cells. 8th AACR-JCA Joint conference, Waikoloa, Hawaii, USA, February 5-9, 2010.
  3. Shing Huang, Pin Ju Chueh, Yun-Wei Lin, Tung-Sheng Shih, Show-Mei Chuang* (2009) Long-term Exposure to nano-TiO2 Promotes Genomic Instability and Cell Transformation in Mammalian Cells. 2009 AACR annual meeting, Denver, NC, USA, April 17-22, 2009.
  4. Show-Mei Chuang, Lyu-Han Wang, Jhao-Hao Hon, Yun-Wei Lin (2008) B[a]p increases Rad51 level and prevents cytotoxicity and mutagenicity in human lung cancer cells. 2008 AACR annual meeting, San Diego, CA, USA, April 12-16, 2008.
  5. Yun-Wei Lin, Chao-Min Cheng, Jen-Chung Ko, Shih-Ci Ciou, Jhih-Yuan Jhan, Ying-Jhen Su, Szu-Ting Lin, and Show-Mei Chuang* (2008) Role of MKK1/2-ERK1/2 and PI3K-AKT signaling in erlotinib decrease Rad51 expression and cell survival in human non-small cell lung cancer cells. 2008 AACR annual meeting, San Diego, CA, USA, April 12-16, 2008.
  6. Huang, S., Chuang, S.-M., Huang, Y-S., and Cheuh, P-J. (2007) Investigation of biological effects of TiO2 nanparticles on mammalian NIH3T3 cells. 3rd International Symposium on Nantechnology, Occupational and Environmental Health. Taipei, Taiwan, Aug. 29 - Sep 1, 2007.
  7. Liu, S., Chuang, S. M., and Chueh, P. J. (2006) The Biological Function of tNOX in Cell Migration by RNAi-mediated Gene Silencing. The American Society for Cell Biology, USA, San Diego, December 8-13.
  8. Kiran Madura, David Lambertson, Show-Mei Chuang, Tatiana G. Ortolan, Li Chen, and Lizbeth Romero-Perez (2004) Genetic studies reveal insight into Rad23 function. FASEB journal 18(8): C309-C309 Suppl. S, May 14, 2004.
  9. Chuang, S.-M., and Yang, J.-L. (2001) Involvement of catalase in regulation of c-JUN N-terminal kinase activation. Mutation Research 483 (Suppl. 1) S139. (8th International Conference on Environmental Mutagens. Shizuoka, Japan, October 21-26, 2001.
  10. Yang, J.-L., Liou, G.-Y., Chuang, S.-M., and Lin, Y.-W. (2001) Roles of three mitogen-activated protein kinases in UV-induced mutagenesis. Mutation Research 483 (Suppl. 1) S89. (8th International Conference on Environmental Mutagens. Shizuoka, Japan, October 21-26, 2001)
  11. Chuang, S.-M., Yang J.-L. (1998) Roles of reactive oxygen species in the activation of JNK, p38, and ERK by chromium(VI) in non-small-cell lung carcinoma cells. Cancer Detection and Prevention 22 (Suppl. 1), Page S-60 (4th International Symposium on Predictive Oncology and Therapy. Nice, France, October 24-27, 1998)
  12. Chuang, S.-M., Wang, I.-C, Hwua, Y.-S., Yang, J.-L. (1997) Role of JNK activation in genotoxicity induced by cadmium chloride in mammalian cells. Page 34. (American Cancer Society 8th Annual Research Conference. East Lansing, Michigan, September 12-13, 1997)
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